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High-sensitivity ChIP kit is a complete set of optimized reagents to carry out a successful chromatin immunoprecipitation procedure in a high throughput format starting from mammalian cells or tissues. - Cited in over 40 publications - Optimized to reduce non-specific background - Enables detection of low-abundance targets - Compatible with PCR, microarrays & sequencing
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4) The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family Transcripts from this gene lack polyA tails instead they contain a palindromic termination element This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21 3
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Because polystyrene transfers sonic wave energy much more efficiently than polypropylene, Active Motif highly recommends that its Polystyrene Sonication Tubes be used with its Q800R Sonicator. Use of polypropylene tubes in indirect sonication has been shown to reduce the extent to which the chromatin is sheared
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